HIV RNA nano-sensors for detection of the minimal residual disease, single-cell imaging of latent infection, and RNA-guided nano-therapeutics of AIDS
Dear friends and colleagues: I am delighted to be able to let you know that very important article entitled “Activation of endogenous human stem cell-associated retroviruses (SCARs) and therapy-resistant phenotypes of malignant tumors” just has been published online in its final form. Below please find the FREE link access to this article. The FREE link is valid for 50 days only.
This is fascinating story to read. It is presenting the multiple lines of experimental and clinical evidence that firmly established the link between the SCARs activation, emergence of cancer stem cells, and development of therapy-resistant sub-types of human malignancies diagnosed in multiple organs. It also outlines new diagnostic and therapeutic strategies that specifically tailored for successful diagnosis, targeting, and eventual eradication of this lethal form of malignant disease. At least some of these therapies may be immediately available and as pointed out in the paper “…may be considered for cancer patients with advanced metastatic disease who do not have other viable options in their fight against these devastating maladies.”
Enjoy the reading and studying this work. The path to victory in our war against cancer is clear. I sincerely hope that you will join us in our life-long fight against terrible diseases that devastated so many families and killed so many of our friends. I would like to encourage you to help us in any way you can to dramatically change the way we think about how cancer develops and how to successfully diagnose, prevent, and treat metastatic disease.
NANO-SENSORS FOR SINGLE-NUCLEOTIDE-RESOLUTION NUCLEIC ACIDS DETECTION AND DISCRIMINATION
US Provisional Patent Application
Disclosed are nano-sensors and techniques for their design, engineering, and use for detection and discrimination of nucleic acids with a single nucleotide resolution. In some implementations, disclosed nano-sensors have achieved ~200,000-fold enhancement of sensitivity for detection of RNA molecules compared to current technologies. Applications include tools for biological and biomedical research and diagnostic, prognostic, and therapeutic applications, including a single-cell imaging applications, for personalized chemoprevention and disease management (e.g., for cancers, cardiovascular and neurodegenerative disorders, diabetes, neurological and developmental disorders). The disclosed technology relies on architectural principles, based on DNA strand displacement reactions and biologically relevant mechanisms of DNA strand dissociation and re-association, to design and implement analytical nano-devices uses. In one aspect, it hypothesizes that regulatory mechanisms affecting DNA dynamics in vivo involve protein-less structural-functional determinants, in particular, interactions of DNA strands with invading RNA molecules. The nano-sensors design techniques utilize conversion of a chemical cue into a substantial mechanical strand displacement detected by time-lapse fluorescence measurements and imaging techniques. The disclosed technology is ready for the immediate implementation in vitro.
This patent document relates to nano-sensors and techniques for their design, engineering, and use for detection and discrimination of nucleic acids with a single nucleotide resolution.
Full text of patent application is available upon request.